Interaction of Cu(II) and Ni(II) with Ypk9 protein fragment via NMR studies.

ABSTRACT: P₁D₂E₃K₄H₅E₆L₇ (PK9-H), a fragment of Ypk9, the yeast homologue of the human Park9 protein, was studied for its coordination abilities towards Ni(II) and Cu(II) ions through mono- and bi-dimensional NMR techniques. Both proteins are involved in the transportation of metal ions, including manganese and nickel, from the cytosol to the lysosomal lumen. Ypk9 showed manganese detoxification role, preventing a Mn-induced Parkinsonism (PD) besides mutations in Park9, linked to a juvenile form of the disease. Here, we tested PK9-H with Cu(II) and Ni(II) ions, the former because it is an essential element ubiquitous in the human body, so its trafficking should be strictly regulated and one cannot exclude that Ypk9 may play a role in it, and the latter because, besides being a toxic element for many

SUBMITTER: Peana MF 

PROVIDER: S-EPMC3982466 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature