Template switching during break-induced replication is promoted by the Mph1 helicase in Saccharomyces cerevisiae.

ABSTRACT: Chromosomal double-strand breaks (DSBs) that have only one end with homology to a donor duplex undergo repair by strand invasion followed by replication to the chromosome terminus (break-induced replication, BIR). Using a transformation-based assay system, it was previously shown that BIR could occur by several rounds of strand invasion, DNA synthesis, and dissociation. Here we describe a modification of the transformation-based assay to facilitate detection of switching between donor templates during BIR by genetic selection in diploid yeast. In addition to the expected recovery of template switch products, we found a high frequency of recombination between chromosome homologs during BIR, suggesting transfer of the DSB from the transforming linear DNA to the donor chromosome, initiating s

SUBMITTER: Stafa A 

PROVIDER: S-EPMC3982708 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature