Gcn5 and sirtuins regulate acetylation of the ribosomal protein transcription factor Ifh1.

ABSTRACT:

Background

In eukaryotes, ribosome biosynthesis involves the coordination of ribosomal RNA and ribosomal protein (RP) production. In S. cerevisiae, the regulation of ribosome biosynthesis occurs largely at the level of transcription. The transcription factor Ifh1 binds at RP genes and promotes their transcription when growth conditions are favorable. Although Ifh1 recruitment to RP genes has been characterized, little is known about the regulation of promoter-bound Ifh1.

Results

We used a novel whole-cell-extract screening approach to identify Spt7, a member of the SAGA transcription complex, and the RP transactivator Ifh1 as highly acetylated nonhistone species. We report that Ifh1 is modified by acetylation specifically in an N-terminal domain. These acetylations require t

SUBMITTER: Downey M 

PROVIDER: S-EPMC3982851 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature