Superoxide dismutase expression attenuates cigarette smoke- or elastase-generated emphysema in mice.

ABSTRACT:

Rationale

Oxidants are believed to play a major role in the development of emphysema.

Objectives

This study aimed to determine if the expression of human copper-zinc superoxide dismutase (CuZnSOD) within the lungs of mice protects against the development of emphysema.

Methods

Transgenic CuZnSOD and littermate mice were exposed to cigarette smoke (6 h/d, 5 d/wk, for 1 yr) and compared with nonexposed mice. A second group was treated with intratracheal elastase to induce emphysema.

Measurements

Lung inflammation was measured by cell counts and myeloperoxidase levels. Oxidative damage was assessed by immunofluorescence for 3-nitrotyrosine and 8-hydroxydeoxyguanosine and lipid peroxidation levels. The development of emphysema was determined by measuring the mean li

SUBMITTER: Foronjy RF 

PROVIDER: S-EPMC3982860 | biostudies-literature | 2006 Mar

REPOSITORIES: biostudies-literature