Combining amine metabolomics and quantitative proteomics of cancer cells using derivatization with isobaric tags.

ABSTRACT: Quantitative metabolomics and proteomics technologies are powerful approaches to explore cellular metabolic regulation. Unfortunately, combining the two technologies typically requires different LC-MS setups for sensitive measurement of metabolites and peptides. One approach to enhance the analysis of certain classes of metabolites is by derivatization with various types of tags to increase ionization and chromatographic efficiency. We demonstrate here that derivatization of amine metabolites with tandem mass tags (TMT), typically used in multiplexed peptide quantitation, facilitates amino acid analysis by standard nanoflow reversed-phase LC-MS setups used for proteomics. We demonstrate that this approach offers the potential to perform experiments at the MS1-level using duplex tags or at

SUBMITTER: Murphy JP 

PROVIDER: S-EPMC3983006 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature