Kinetic and equilibrium binding characterization of aptamers to small molecules using a label-free, sensitive, and scalable platform.

ABSTRACT: Nucleic acid aptamers function as versatile sensing and targeting agents for analytical, diagnostic, therapeutic, and gene-regulatory applications, but their limited characterization and functional validation have hindered their broader implementation. We report the development of a surface plasmon resonance-based platform for rapid characterization of kinetic and equilibrium binding properties of aptamers to small molecules. Our system is label-free and scalable and enables analysis of different aptamer-target pairs and binding conditions with the same platform. This method demonstrates improved sensitivity, flexibility, and stability compared to other aptamer characterization methods. We validated our assay against previously reported aptamer affinity and kinetic measurements and further

SUBMITTER: Chang AL 

PROVIDER: S-EPMC3983011 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature