Anions in electrothermal supercharging of proteins with electrospray ionization follow a reverse Hofmeister series.

ABSTRACT: The effects of different anions on the extent of electrothermal supercharging of proteins from aqueous ammonium and sodium salt solutions were investigated. Sulfate and hydrogen phosphate are the most effective anions at producing high charge state protein ions from buffered aqueous solution, whereas iodide and perchlorate are ineffective with electrothermal supercharging. The propensity for these anions to produce high charge state protein ions follows the following trend: sulfate > hydrogen phosphate > thiocyanate > bicarbonate > chloride > formate ≈ bromide > acetate > iodide > perchlorate. This trend correlates with the reverse Hofmeister series over a wide range of salt concentrations (1 mM to 2 M) and with several physical properties, including solvent surface tension, anion viscosit

SUBMITTER: Cassou CA 

PROVIDER: S-EPMC3983018 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature