Methylome diversification through changes in DNA methyltransferase sequence specificity.

ABSTRACT: Epigenetic modifications such as DNA methylation have large effects on gene expression and genome maintenance. Helicobacter pylori, a human gastric pathogen, has a large number of DNA methyltransferase genes, with different strains having unique repertoires. Previous genome comparisons suggested that these methyltransferases often change DNA sequence specificity through domain movement--the movement between and within genes of coding sequences of target recognition domains. Using single-molecule real-time sequencing technology, which detects N6-methyladenines and N4-methylcytosines with single-base resolution, we studied methylated DNA sites throughout the H. pylori genome for several closely related strains. Overall, the methylome was highly variable among closely related strains. Hyperme

SUBMITTER: Furuta Y 

PROVIDER: S-EPMC3983042 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature