Transcriptome analysis of the Portunus trituberculatus: de novo assembly, growth-related gene identification and marker discovery.

ABSTRACT:

Background

The swimming crab, Portunus trituberculatus, is an important farmed species in China, has been attracting extensive studies, which require more and more genome background knowledge. To date, the sequencing of its whole genome is unavailable and transcriptomic information is also scarce for this species. In the present study, we performed de novo transcriptome sequencing to produce a comprehensive transcript dataset for major tissues of Portunus trituberculatus by the Illumina paired-end sequencing technology.

Results

Total RNA was isolated from eyestalk, gill, heart, hepatopancreas and muscle. Equal quantities of RNA from each tissue were pooled to construct a cDNA library. Using the Illumina paired-end sequencing technology, we generated a total of 120,137 transc

SUBMITTER: Lv J 

PROVIDER: S-EPMC3983128 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature