Transcriptomic analysis of Petunia hybrida in response to salt stress using high throughput RNA sequencing.

ABSTRACT: Salinity and drought stress are the primary cause of crop losses worldwide. In sodic saline soils sodium chloride (NaCl) disrupts normal plant growth and development. The complex interactions of plant systems with abiotic stress have made RNA sequencing a more holistic and appealing approach to study transcriptome level responses in a single cell and/or tissue. In this work, we determined the Petunia transcriptome response to NaCl stress by sequencing leaf samples and assembling 196 million Illumina reads with Trinity software. Using our reference transcriptome we identified more than 7,000 genes that were differentially expressed within 24 h of acute NaCl stress. The proposed transcriptome can also be used as an excellent tool for biological and bioinformatics in the absence of an availab

SUBMITTER: Villarino GH 

PROVIDER: S-EPMC3983219 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature