Efficient induction of productive Cre-mediated recombination in retinal pigment epithelium.

ABSTRACT:

Purpose

To dissect gene functions in the retinal pigment epithelium (RPE), we previously generated a tetracycline-inducible RPE-specific Cre mouse line. Although this Cre mouse line was useful for several conditional gene targeting studies that were conducted by different laboratories, its potential has not been fully exploited, presumably due to a lack of knowledge or procedure for inducing Cre expression appropriately in this mouse line. The goal of the current study is to establish a procedure that will improve the reproducibility of Cre-mediated recombination in this mouse line.

Methods

Analysis of Cre expression and function was performed in double transgenic mice derived from inducible RPE-specific Cre and Cre-activatable ROSA26 lacZ reporter mice. A tetracycline deriv

SUBMITTER: Fu S 

PROVIDER: S-EPMC3984040 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature