Hybridization-based antibody cDNA recovery for the production of recombinant antibodies identified by repertoire sequencing.

ABSTRACT: High-throughput sequencing of the antibody repertoire is enabling a thorough analysis of B cell diversity and clonal selection, which may improve the novel antibody discovery process. Theoretically, an adequate bioinformatic analysis could allow identification of candidate antigen-specific antibodies, requiring their recombinant production for experimental validation of their specificity. Gene synthesis is commonly used for the generation of recombinant antibodies identified in silico. Novel strategies that bypass gene synthesis could offer more accessible antibody identification and validation alternatives. We developed a hybridization-based recovery strategy that targets the complementarity-determining region 3 (CDRH3) for the enrichment of cDNA of candidate antigen-specific antibody seq

SUBMITTER: Valdes-Aleman J 

PROVIDER: S-EPMC3984337 | biostudies-literature | 2014 Mar-Apr

REPOSITORIES: biostudies-literature