Comparative reactivity of human IgE to cynomolgus monkey and human effector cells and effects on IgE effector cell potency.

ABSTRACT:

Background

Due to genetic similarities with humans, primates of the macaque genus such as the cynomolgus monkey are often chosen as models for toxicology studies of antibody therapies. IgE therapeutics in development depend upon engagement with the FcεRI and FcεRII receptors on immune effector cells for their function. Only limited knowledge of the primate IgE immune system is available to inform the choice of models for mechanistic and safety evaluations.

Methods

The recognition of human IgE by peripheral blood lymphocytes from cynomolgus monkey and man was compared. We used effector cells from each species in ex vivo affinity, dose-response, antibody-receptor dissociation and potency assays.

Results

We report cross-reactivity of human IgE Fc with cynomolgus monkey c

SUBMITTER: Saul L 

PROVIDER: S-EPMC3984339 | biostudies-literature | 2014 Mar-Apr

REPOSITORIES: biostudies-literature