Sustained elevation of Snail promotes glial-mesenchymal transition after irradiation in malignant glioma.

ABSTRACT:

Background

Ionizing irradiation is an effective treatment for malignant glioma (MG); however, a higher rate of recurrence with more aggressive phenotypes is a vital issue. Although epithelial-mesenchymal transition (EMT) is involved in irradiation-induced cancer progression, the role for such phenotypic transition in MG remains unknown.

Methods

To investigate the mechanism of irradiation-dependent tumor progression in MG, we performed immunohistochemistry (IHC) and qRT-PCR using primary and recurrent MG specimens, MG cell lines, and primary culture cells of MG. siRNA technique was used for MG cell lines.

Results

In 22 cases of clinically recurrent MG, the expression of the mesenchymal markers vimentin and CD44 was found to be increased by IHC. In paired identical MG o

SUBMITTER: Mahabir R 

PROVIDER: S-EPMC3984547 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature