Inhibition of HSP70 reduces porcine reproductive and respiratory syndrome virus replication in vitro.

ABSTRACT:

Background

Successful viral infection requires the involvement of host cellular factors in their life cycle. Heat shock protein 70 (HSP70) can be recruited by numerous viruses to promote the folding, maturation, or assembly of viral proteins. We have previously shown that HSP70 is significantly elevated in porcine reproductive and respiratory syndrome virus (PRRSV)-infected lungs, suggesting HSP70 may play a potential role during PRRSV infection. In this study, we tried to investigate the role of HSP70 during PRRSV infection.

Results

In this study, we observed that PRRSV infection induced the expression of HSP70. The down-regulation of HSP70 using quercetin, a HSPs synthesis inhibitor, or small interfering RNAs (siRNA) reduced the viral protein level and viral production. No

SUBMITTER: Gao J 

PROVIDER: S-EPMC3984673 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature