Engineering of three-dimensional microenvironments to promote contractile behavior in primary intestinal organoids.

ABSTRACT: Multiple culture techniques now exist for the long-term maintenance of neonatal primary murine intestinal organoids in vitro; however, the achievement of contractile behavior within cultured organoids has thus far been infrequent and unpredictable. Here we combine finite element simulation of oxygen transport and quantitative comparative analysis of cellular microenvironments to elucidate the critical variables that promote reproducible intestinal organoid contraction. Experimentally, oxygen distribution was manipulated by adjusting the ambient oxygen concentration along with the use of semi-permeable membranes to enhance transport. The culture microenvironment was further tailored through variation of collagen type-I matrix density, addition of exogenous R-spondin1, and specification of c

SUBMITTER: DiMarco RL 

PROVIDER: S-EPMC3984951 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature