Base pairing configuration and stability of an oligonucleotide duplex containing a 5-chlorouracil-adenine base pair.

ABSTRACT: Inflammation-mediated reactive molecules can damage DNA by oxidation and chlorination. The biological consequences of this damage are as yet incompletely understood. In this paper, we have constructed oligonucleotides containing 5-chlorouracil (ClU), one of the known inflammation damage products. The thermodynamic stability, base pairing configuration, and duplex conformation of oligonucleotides containing ClU paired opposite adenine have been examined. NMR spectra reveal that the ClU-A base pair adopts a geometry similar to that of the T-A base pair, and the ClU-A base pair-containing duplex adopts a normal B-form conformation. The line width of the imino proton of the ClU residue is substantially greater than that of the corresponding T imino proton; however, this difference is not attri

SUBMITTER: Theruvathu JA 

PROVIDER: S-EPMC3985277 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature