Mapping of six somatic linker histone H1 variants in human breast cancer cells uncovers specific features of H1.2.

ABSTRACT: Seven linker histone H1 variants are present in human somatic cells with distinct prevalence across cell types. Despite being key structural components of chromatin, it is not known whether the different variants have specific roles in the regulation of nuclear processes or are differentially distributed throughout the genome. Using variant-specific antibodies to H1 and hemagglutinin (HA)-tagged recombinant H1 variants expressed in breast cancer cells, we have investigated the distribution of six H1 variants in promoters and genome-wide. H1 is depleted at promoters depending on its transcriptional status and differs between variants. Notably, H1.2 is less abundant than other variants at the transcription start sites of inactive genes, and promoters enriched in H1.2 are different from those

SUBMITTER: Millan-Arino L 

PROVIDER: S-EPMC3985652 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature