Oxidative stress interferes with white matter renewal after prolonged cerebral hypoperfusion in mice.

ABSTRACT:

Background and purpose

White matter injury caused by cerebral hypoperfusion may contribute to the pathophysiology of vascular dementia and stroke, but the underlying mechanisms remain to be fully defined. Here, we test the hypothesis that oxidative stress interferes with endogenous white matter repair by disrupting renewal processes mediated by oligodendrocyte precursor cells (OPCs).

Methods

In vitro, primary rat OPCs were exposed to sublethal CoCl2 for 7 days to induce prolonged chemical hypoxic stress. Then, OPC proliferation/differentiation was assessed. In vivo, prolonged cerebral hypoperfusion was induced by bilateral common carotid artery stenosis in mice. Then, reactive oxygen species production, myelin density, oligodendrocyte versus OPC counts, and cognitive functio

SUBMITTER: Miyamoto N 

PROVIDER: S-EPMC3985753 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature