Mice with megabase humanization of their immunoglobulin genes generate antibodies as efficiently as normal mice.

ABSTRACT: Mice genetically engineered to be humanized for their Ig genes allow for human antibody responses within a mouse background (HumAb mice), providing a valuable platform for the generation of fully human therapeutic antibodies. Unfortunately, existing HumAb mice do not have fully functional immune systems, perhaps because of the manner in which their genetic humanization was carried out. Heretofore, HumAb mice have been generated by disrupting the endogenous mouse Ig genes and simultaneously introducing human Ig transgenes at a different and random location; KO-plus-transgenic humanization. As we describe in the companion paper, we attempted to make mice that more efficiently use human variable region segments in their humoral responses by precisely replacing 6 Mb of mouse Ig heavy and kappa

SUBMITTER: Murphy AJ 

PROVIDER: S-EPMC3986188 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature