A modified screening system for loss-of-function and dominant negative alleles of essential MCMV genes.

ABSTRACT: Inactivation of gene products by dominant negative mutants is a valuable tool to assign functions to yet uncharacterized proteins, to map protein-protein interactions or to dissect physiological pathways. Detailed functional and structural knowledge about the target protein would allow the construction of inhibitory mutants by targeted mutagenesis. Yet, such data are limited for the majority of viral proteins, so that the target gene needs to be subjected to random mutagenesis to identify suitable mutants. However, for cytomegaloviruses this requires a two-step screening approach, which is time-consuming and labor-intensive. Here, we report the establishment of a high-throughput suitable screening system for the identification of inhibitory alleles of essential genes of the murine cytomega

SUBMITTER: Pogoda M 

PROVIDER: S-EPMC3986410 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature