Cell death stages in single apoptotic and necrotic cells monitored by Raman microspectroscopy.

ABSTRACT: Although apoptosis and necrosis have distinct features, the identification and discrimination of apoptotic and necrotic cell death in vitro is challenging. Immunocytological and biochemical assays represent the current gold standard for monitoring cell death pathways; however, these standard assays are invasive, render large numbers of cells and impede continuous monitoring experiments. In this study, both room temperature (RT)-induced apoptosis and heat-triggered necrosis were analyzed in individual Saos-2 and SW-1353 cells by utilizing Raman microspectroscopy. A targeted analysis of defined cell death modalities, including early and late apoptosis as well as necrosis, was facilitated based on the combination of Raman spectroscopy with fluorescence microscopy. Spectral shifts were identif

SUBMITTER: Brauchle E 

PROVIDER: S-EPMC3986703 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature