High-throughput transcriptome sequencing and preliminary functional analysis in four Neotropical tree species.

ABSTRACT:

Background

The Amazonian rainforest is predicted to suffer from ongoing environmental changes. Despite the need to evaluate the impact of such changes on tree genetic diversity, we almost entirely lack genomic resources.

Results

In this study, we analysed the transcriptome of four tropical tree species (Carapa guianensis, Eperua falcata, Symphonia globulifera and Virola michelii) with contrasting ecological features, belonging to four widespread botanical families (respectively Meliaceae, Fabaceae, Clusiaceae and Myristicaceae). We sequenced cDNA libraries from three organs (leaves, stems, and roots) using 454 pyrosequencing. We have developed an R and bioperl-based bioinformatic procedure for de novo assembly, gene functional annotation and marker discovery. Mismatch identi

SUBMITTER: Brousseau L 

PROVIDER: S-EPMC3986928 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature