Peroxidase gene discovery from the horseradish transcriptome.

ABSTRACT:

Background

Horseradish peroxidases (HRPs) from Armoracia rusticana have long been utilized as reporters in various diagnostic assays and histochemical stainings. Regardless of their increasing importance in the field of life sciences and suggested uses in medical applications, chemical synthesis and other industrial applications, the HRP isoenzymes, their substrate specificities and enzymatic properties are poorly characterized. Due to lacking sequence information of natural isoenzymes and the low levels of HRP expression in heterologous hosts, commercially available HRP is still extracted as a mixture of isoenzymes from the roots of A. rusticana.

Results

In this study, a normalized, size-selected A. rusticana transcriptome library was sequenced using 454 Titanium technology

SUBMITTER: Naatsaari L 

PROVIDER: S-EPMC3987668 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature