Influence of speed of sample processing on placental energetics and signalling pathways: implications for tissue collection.

ABSTRACT:

Introduction

The placenta is metabolically highly active due to extensive endocrine and active transport functions. Hence, placental tissues soon become ischaemic after separation from the maternal blood supply. Ischaemia rapidly depletes intracellular ATP, and leads to activation of stress-response pathways aimed at reducing metabolic demands and conserving energy resources for vital functions. Therefore, this study aimed to elucidate the effects of ischaemia ex vivo as may occur during tissue collection on phosphorylation of placental proteins and kinases involved in growth and cell survival, and on mitochondrial complexes.

Methods

Eight term placentas obtained from normotensive non-laboured elective caesarean sections were kept at room-temperature and sampled at 10, 20, 3

SUBMITTER: Yung HW 

PROVIDER: S-EPMC3988954 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature