Large-scale development of gene-associated single-nucleotide polymorphism markers for molluscan population genomic, comparative genomic, and genome-wide association studies.

ABSTRACT: Mollusca is the second most diverse group of animals in the world. Despite their perceived importance, omics-level studies have seldom been applied to this group of animals largely due to a paucity of genomic resources. Here, we report the first large-scale gene-associated marker development and evaluation for a bivalve mollusc, Chlamys farreri. More than 21,000 putative single-nucleotide polymorphisms (SNPs) were identified from the C. farreri transcriptome. Primers and probes were designed and synthesized for 4500 SNPs, and 1492 polymorphic markers were successfully developed using a high-resolution melting genotyping platform. These markers are particularly suitable for population genomic analysis due to high polymorphism within and across populations, a low frequency of null alleles, a

SUBMITTER: Jiao W 

PROVIDER: S-EPMC3989488 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature