Toll-like receptor 7-stimulated tumor necrosis factor α causes bone marrow damage in systemic lupus erythematosus.

ABSTRACT:

Objective

To define the pathogenesis of bone marrow (BM) involvement in systemic lupus erythematosus (SLE).

Methods

Tumor necrosis factor α (TNFα) levels, cell death, and cellular damage in BM from SLE patients, controls, and mice with pristane-induced lupus were analyzed using a morphometric technique and immunohistochemistry. The pathogenesis of BM abnormalities was studied in wild-type (WT), TNFα(-/-) , Toll-like receptor-deficient (TLR-7(-/-) ), interferon (IFN)-α/β/ω receptor-knockout (IFNAR(-/-) ), and B cell-deficient (μmt) mice treated with pristane. Flow cytometry was used to examine TNFα production (by intracellular staining) and plasma cell/plasmablast development. CXCL12 expression was determined by quantitative polymerase chain reaction.

Results

BM from S

SUBMITTER: Zhuang H 

PROVIDER: S-EPMC3990233 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature