Disruption of the homogentisate solanesyltransferase gene results in albino and dwarf phenotypes and root, trichome and stomata defects in Arabidopsis thaliana.

ABSTRACT: Homogentisate solanesyltransferase (HST) plays an important role in plastoquinone (PQ) biosynthesis and acts as the electron acceptor in the carotenoids and abscisic acid (ABA) biosynthesis pathways. We isolated and identified a T-DNA insertion mutant of the HST gene that displayed the albino and dwarf phenotypes. PCR analyses and functional complementation also confirmed that the mutant phenotypes were caused by disruption of the HST gene. The mutants also had some developmental defects, including trichome development and stomata closure defects. Chloroplast development was also arrested and chlorophyll (Chl) was almost absent. Developmental defects in the chloroplasts were consistent with the SDS-PAGE result and the RNAi transgenic phenotype. Exogenous gibberellin (GA) could partially re

SUBMITTER: Chao Y 

PROVIDER: S-EPMC3990575 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature