Conditional reverse tet-transactivator mouse strains for the efficient induction of TRE-regulated transgenes in mice.

ABSTRACT: Tetracycline or doxycycline (dox)-regulated control of genetic elements allows inducible, reversible and tissue specific regulation of gene expression in mice. This approach provides a means to investigate protein function in specific cell lineages and at defined periods of development and disease. Efficient and stable regulation of cDNAs or non-coding elements (e.g. shRNAs) downstream of the tetracycline-regulated element (TRE) requires the robust expression of a tet-transactivator protein, commonly the reverse tet-transactivator, rtTA. Most rtTA strains rely on tissue specific promoters that often do not provide sufficient rtTA levels for optimal inducible expression. Here we describe the generation of two mouse strains that enable Cre-dependent, robust expression of rtTA3, providing tis

SUBMITTER: Dow LE 

PROVIDER: S-EPMC3990578 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature