A new tool improves diagnostic test performance for transmission em evaluation of axonemal dynein arms.

ABSTRACT: Abstract Diagnosis of primary ciliary dyskinesia (PCD) by identification of dynein arm loss in transmission electron microscopy (TEM) images can be confounded by high background noise due to random electron-dense material within the ciliary matrix, leading to diagnostic uncertainty even for experienced morphologists. The authors developed a novel image analysis tool to average the axonemal peripheral microtubular doublets, thereby increasing microtubular signal and reducing random background noise. In a randomized, double-blinded study that compared two experienced morphologists and three different diagnostic approaches, they found that use of this tool led to improvement in diagnostic TEM test performance.

SUBMITTER: Funkhouser WK 

PROVIDER: S-EPMC3990650 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature