Surrogate production of eggs and sperm by intrapapillary transplantation of germ cells in cytoablated adult fish.

ABSTRACT: Germ cell transplantation (GCT) is a promising assisted reproductive technology for the conservation and propagation of endangered and valuable genetic resources. In teleost fish, GCT in adult gonads has been achieved only in male recipients, limiting greatly the usefulness of this technique in situations where both sexes need equal and timely attention for conservation and/or propagation. Here we describe a simplified GCT approach that ultimately leads to production of donor-derived eggs and sperm in considerably short time. Donor germ cells isolated from young pejerrey Odontesthes bonariensis (Atherinopsidae) were transplanted non-surgically through the genital papilla into the sexually mature gonads of Patagonian pejerrey O. hatcheri recipients whose gonads have been depleted of endogen

SUBMITTER: Majhi SK 

PROVIDER: S-EPMC3991631 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature