Identification of transcriptional and metabolic programs related to mammalian cell size.

ABSTRACT:

Background

Regulation of cell size requires coordination of growth and proliferation. Conditional loss of cyclin-dependent kinase 1 in mice permits hepatocyte growth without cell division, allowing us to study cell size in vivo using transcriptomics and metabolomics.

Results

Larger cells displayed increased expression of cytoskeletal genes but unexpectedly repressed expression of many genes involved in mitochondrial functions. This effect appears to be cell autonomous because cultured Drosophila cells induced to increase cell size displayed a similar gene-expression pattern. Larger hepatocytes also displayed a reduction in the expression of lipogenic transcription factors, especially sterol-regulatory element binding proteins. Inhibition of mitochondrial functions and lipid

SUBMITTER: Miettinen TP 

PROVIDER: S-EPMC3991852 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature