Differential expression of genes in cells cultured from juxtacanalicular trabecular meshwork and Schlemm's canal.

ABSTRACT:

Purpose

The purpose of this study was to distinguish differences in gene expression between cells cultured from the juxtacanalicular trabecular meshwork (JCTM) and those from Schlemm's canal (SC), to gain clues to differences between those cell types, and to add to our baseline knowledge of gene expression differences in these cell types for later comparison between cells from nonprimary open-angle glaucoma (POAG) and POAG outflow tissues.

Methods

A set of JCTM and SC cells was cultured from each of 2 donor eyes by an explant method, grown to passage 3, and frozen in liquid nitrogen. The cells were thawed, total RNA was extracted, and the probes made from total RNAs were hybridized to MICROMAX human cDNA microarray slides in 2 separate trials. Differentially expressed genes

SUBMITTER: O'Brien ET 

PROVIDER: S-EPMC3991976 | biostudies-literature | 2014 Mar-Apr

REPOSITORIES: biostudies-literature