Analyzing protein dynamics using hydrogen exchange mass spectrometry.

ABSTRACT: All cellular processes depend on the functionality of proteins. Although the functionality of a given protein is the direct consequence of its unique amino acid sequence, it is only realized by the folding of the polypeptide chain into a single defined three-dimensional arrangement or more commonly into an ensemble of interconverting conformations. Investigating the connection between protein conformation and its function is therefore essential for a complete understanding of how proteins are able to fulfill their great variety of tasks. One possibility to study conformational changes a protein undergoes while progressing through its functional cycle is hydrogen-(1)H/(2)H-exchange in combination with high-resolution mass spectrometry (HX-MS). HX-MS is a versatile and robust method that add

SUBMITTER: Hentze N 

PROVIDER: S-EPMC3992118 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature