Biomarkers of coordinate metabolic reprogramming in colorectal tumors in mice and humans.

ABSTRACT:

Background & aims

There are no robust noninvasive methods for colorectal cancer screening and diagnosis. Metabolomic and gene expression analyses of urine and tissue samples from mice and humans were used to identify markers of colorectal carcinogenesis.

Methods

Mass spectrometry-based metabolomic analysis of urine and tissues from wild-type C57BL/6J and Apc(Min/+) mice, as well as from mice with azoxymethane-induced tumors, was employed in tandem with gene expression analysis. Metabolic profiling was also performed on colon tumor and adjacent nontumor tissues from 39 patients. The effects of β-catenin activity on metabolic profiles were assessed in mice with colon-specific disruption of Apc.

Results

Thirteen markers were found in urine associated with development of

SUBMITTER: Manna SK 

PROVIDER: S-EPMC3992178 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature