Activation of the PI3K/AKT pathway correlates with prognosis in stage II colon cancer.

ABSTRACT:

Background

Patients with UICC/AJCC stage II colon cancer have a high 5-year overall survival rate after surgery. Nevertheless, a significant subgroup of patients develops tumour recurrence. Currently, there are no clinically established biomarkers available to identify this patient group. We applied reverse-phase protein arrays (RPPA) for phosphatidylinositide-3-kinase pathway activation mapping to stratify patients according to their risk of tumour recurrence after surgery.

Methods

Full-length proteins were extracted from formalin-fixed, paraffin-embedded tissue samples of 118 patients who underwent curative resection. RPPA technology was used to analyse expression and/or phosphorylation levels of six major factors of the phosphatidylinositide-3-kinase pathway. Oncogenic mu

SUBMITTER: Malinowsky K 

PROVIDER: S-EPMC3992486 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature