Identifying microRNAs regulating B7-H3 in breast cancer: the clinical impact of microRNA-29c.

ABSTRACT:

Background

B7-H3, an immunoregulatory protein, is overexpressed in several cancers and is often associated with metastasis and poor prognosis. Here, our aim was to identify microRNAs (miRNAs) regulating B7-H3 and assess their potential prognostic implications in breast cancer.

Methods

MicroRNAs targeting B7-H3 were identified by transfecting two breast cancer cell lines with a library of 810 miRNA mimics and quantifying changes of B7-H3 protein levels using protein lysate microarrays. For validations we used western immunoblotting and 3'-UTR luciferase assays. Clinical significance of the miRNAs was assayed by analysing whether their expression levels correlated with outcome in two cohorts of breast cancer patients (142 and 81 patients).

Results

We identified nearly 5

SUBMITTER: Nygren MK 

PROVIDER: S-EPMC3992492 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature