ERRβ signalling through FST and BCAS2 inhibits cellular proliferation in breast cancer cells.

ABSTRACT:

Background

The overexpression of oestrogen-related receptor-β (ERRβ) in breast cancer patients is correlated with improved prognosis and longer relapse-free survival, and the level of ERRβ mRNA is inversely correlated with the S-phase fraction of cells from breast cancer patients.

Methods

Chromatin immunoprecipitation (ChIP) cloning of ERRβ transcriptional targets and gel supershift assays identified breast cancer amplified sequence 2 (BCAS2) and Follistatin (FST) as two important downstream genes that help to regulate tumourigenesis. Confocal microscopy, co-immunoprecipitation (CoIP), western blotting and quantitative real-time PCR confirmed the involvement of ERRβ in oestrogen signalling.

Results

Overexpressed ERRβ induced FST-mediated apoptosis in breast cancer cel

SUBMITTER: Sengupta D 

PROVIDER: S-EPMC3992508 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature