Imaging bacterial peptidoglycan with near-infrared fluorogenic azide probes.

ABSTRACT: Fluorescent probes designed for activation by bioorthogonal chemistry have enabled the visualization of biomolecules in living systems. Such activatable probes with near-infrared (NIR) emission would be ideal for in vivo imaging but have proven difficult to engineer. We present the development of NIR fluorogenic azide probes based on the Si-rhodamine scaffold that undergo a fluorescence enhancement of up to 48-fold upon reaction with terminal or strained alkynes. We used the probes for mammalian cell surface imaging and, in conjunction with a new class of cyclooctyne D-amino acids, for visualization of bacterial peptidoglycan without the need to wash away unreacted probe.

SUBMITTER: Shieh P 

PROVIDER: S-EPMC3992625 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature