Fibroblast growth factor 2 drives changes in gene expression following injury to murine cartilage in vitro and in vivo.

ABSTRACT:

Objective

The articular cartilage is known to be highly mechanosensitive, and a number of mechanosensing mechanisms have been proposed as mediators of the cellular responses to altered mechanical load. These pathways are likely to be important in tissue homeostasis as well as in the pathogenesis of osteoarthritis. One important injury-activated pathway involves the release of pericellular fibroblast growth factor 2 (FGF-2) from the articular cartilage. Using a novel model of murine cartilage injury and surgically destabilized joints in mice, we examined the extent to which FGF-2 contributes to the cellular gene response to injury.

Methods

Femoral epiphyses from 5-week-old wild-type mice were avulsed and cultured in serum-free medium. Explant lysates were Western blotted for

SUBMITTER: Chong KW 

PROVIDER: S-EPMC3992838 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature