Survival of airborne MS2 bacteriophage generated from human saliva, artificial saliva, and cell culture medium.

ABSTRACT: Laboratory studies of virus aerosols have been criticized for generating airborne viruses from artificial nebulizer suspensions (e.g., cell culture media), which do not mimic the natural release of viruses (e.g., from human saliva). The objectives of this study were to determine the effect of human saliva on the infectivity and survival of airborne virus and to compare it with those of artificial saliva and cell culture medium. A stock of MS2 bacteriophage was diluted in one of three nebulizer suspensions, aerosolized, size selected (100 to 450 nm) using a differential mobility analyzer, and collected onto gelatin filters. Uranine was used as a particle tracer. The resulting particle size distribution was measured using a scanning mobility particle sizer. The amounts of infectious virus, t

SUBMITTER: Zuo Z 

PROVIDER: S-EPMC3993287 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature