Two glycine riboswitches activate the glycine cleavage system essential for glycine detoxification in Streptomyces griseus.

ABSTRACT: The glycine cleavage (GCV) system catalyzes the oxidative cleavage of glycine into CO2, NH4(+), and a methylene group, which is accepted by tetrahydrofolate (THF) to form N(5),N(10)-methylene-THF. Streptomyces griseus contains gcvP and the gcvT-gcvH operon, which encode three intrinsic components of the GCV system. We identified the transcriptional start sites of gcvTH and gcvP and found putative glycine riboswitches in their 5' untranslated regions (5' UTRs). The ratios of the transcripts of the gcvT and gcvP coding sequences (CDSs) to those of the respective 5' UTRs were significantly higher in the presence of glycine in the wild-type strain. However, the levels of gcvT and gcvP CDS transcripts were not increased by glycine in the respective 5' UTR deletion mutants. A reporter gene assay

SUBMITTER: Tezuka T 

PROVIDER: S-EPMC3993345 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature