Evaluation of four single-locus markers for Leishmania species discrimination by sequencing.

ABSTRACT: Several genetic markers have been described for discriminating Leishmania species. In most reported cases, one or a few polymorphisms are the basis of species identification, and the methods were validated on a limited number of strains from a particular geographical region. Therefore, most techniques may underestimate the global intraspecies variability and are applicable only in certain areas. In addition, interlaboratory standardization is mostly absent, complicating comparisons among different studies. Here, we compared species typing results from all sequence polymorphisms found in four popular markers that can be applied directly on clinical samples: the miniexon or spliced leader, the internal transcribed spacer of the ribosomal DNA array, the 7SL RNA gene, and the heat shock protei

SUBMITTER: Van der Auwera G 

PROVIDER: S-EPMC3993476 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature