Distribution of nontuberculous mycobacteria by multigene sequence-based typing and clinical significance of isolated strains.

ABSTRACT: Species identification of nontuberculous mycobacteria (NTM) is challenging due to the increasing number of identified NTM species and the lack of standardized testing strategies. The objectives of this study were to investigate the distribution of NTM species recovered from respiratory specimens by multigene sequence-based typing and to evaluate the clinical significance of identified species. Two hundred thirty-two consecutive clinical NTM isolates were subjected to sequencing of multiple genes, including hsp65, rpoB, and 16S-23S rRNA internal transcribed spacer (ITS) sequence. In addition, clinical data from all patients whose specimens had NTM isolates were analyzed to examine clinical virulence and treatment history. Eighteen strains from 227 isolates from 169 patients were successfull

SUBMITTER: Jang MA 

PROVIDER: S-EPMC3993509 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature