Multiplex high-resolution melting analysis as a diagnostic tool for detection of plasmid-mediated AmpC β-lactamase genes.

ABSTRACT: High-resolution melting (HRM) analysis can be a diagnostic tool to evaluate the presence of resistance genes with the added bonus of discriminating sequence modifications. A real-time, multiplex PCR assay using HRM was designed for the detection of plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 96% and 100%, respectively.

SUBMITTER: Geyer CN 

PROVIDER: S-EPMC3993522 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature