Testing and genotyping of high-risk human papillomavirus by the cobas HPV Test and the Hybrid Capture 2 high-risk HPV DNA test using cervical and vaginal samples.

ABSTRACT: The accurate detection and typing of high-risk human papillomavirus (HPV) are critical for cervical cancer screening. The Hybrid Capture 2 (hc2) and cobas HPV tests showed high agreement for cervical samples (94.4%, ?=0.72, n=693) and moderate agreement for vaginal samples (?=0.62, n=108). The HPV16 and HPV18 results were highly consistent between the cobas and Linear Array tests (??0.96, n=197). Three hc2-negative vaginal samples were repeatedly invalid by the cobas test due to ?-globin control failures, highlighting amplification control benefits. No cross-contamination was detected in a challenge experiment.

SUBMITTER: Pyne MT 

PROVIDER: S-EPMC3993637 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature