Identifying Neisseria species by use of the 50S ribosomal protein L6 (rplF) gene.

ABSTRACT: The comparison of 16S rRNA gene sequences is widely used to differentiate bacteria; however, this gene can lack resolution among closely related but distinct members of the same genus. This is a problem in clinical situations in those genera, such as Neisseria, where some species are associated with disease while others are not. Here, we identified and validated an alternative genetic target common to all Neisseria species which can be readily sequenced to provide an assay that rapidly and accurately discriminates among members of the genus. Ribosomal multilocus sequence typing (rMLST) using ribosomal protein genes has been shown to unambiguously identify these bacteria. The PubMLST Neisseria database (http://pubmlst.org/neisseria/) was queried to extract the 53 ribosomal protein gene sequ

SUBMITTER: Bennett JS 

PROVIDER: S-EPMC3993661 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature