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Detection of carbapenemases by real-time PCR and melt curve analysis on the BD Max system.

ABSTRACT: We developed a multiplex SYBR green real-time PCR for the BD Max instrument (BD Diagnostics, Sparks, MD) to detect a panel of carbapenemases. The assay was evaluated with 152 consecutive isolates sent to the German National Reference Laboratory, and 65/65 of the carbapenemase-positive and 87/87 of the carbapenemase-negative strains were identified correctly.

SUBMITTER: Hofko M 

PROVIDER: S-EPMC3993711 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature

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Detection of carbapenemases by real-time PCR and melt curve analysis on the BD Max system.

Hofko Marjeta M   Mischnik Alexander A   Kaase Martin M   Zimmermann Stefan S   Dalpke Alexander H AH  

Journal of clinical microbiology 20140212 5

We developed a multiplex SYBR green real-time PCR for the BD Max instrument (BD Diagnostics, Sparks, MD) to detect a panel of carbapenemases. The assay was evaluated with 152 consecutive isolates sent to the German National Reference Laboratory, and 65/65 of the carbapenemase-positive and 87/87 of the carbapenemase-negative strains were identified correctly. ...[more]

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