Vaccinia virus requires glutamine but not glucose for efficient replication.

ABSTRACT:

Unlabelled

Viruses require host cell metabolism to provide the necessary energy and biosynthetic precursors for successful viral replication. Vaccinia virus (VACV) is a member of the Poxviridae family, and its use as a vaccine enabled the eradication of variola virus, the etiologic agent of smallpox. A global metabolic screen of VACV-infected primary human foreskin fibroblasts suggested that glutamine metabolism is altered during infection. Glutamine and glucose represent the two main carbon sources for mammalian cells. Depriving VACV-infected cells of exogenous glutamine led to a substantial decrease in infectious virus production, whereas starving infected cells of exogenous glucose had no significant impact on replication. Viral yield in glutamine-deprived cells or in cells trea

SUBMITTER: Fontaine KA 

PROVIDER: S-EPMC3993723 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature