Murine leukemia virus uses NXF1 for nuclear export of spliced and unspliced viral transcripts.

ABSTRACT:

Unlabelled

Intron-containing mRNAs are subject to restricted nuclear export in higher eukaryotes. Retroviral replication requires the nucleocytoplasmic transport of both spliced and unspliced RNA transcripts, and RNA export mechanisms of gammaretroviruses are poorly characterized. Here, we report the involvement of the nuclear export receptor NXF1/TAP in the nuclear export of gammaretroviral RNA transcripts. We identified a conserved cis-acting element in the pol gene of gammaretroviruses, including murine leukemia virus (MLV) and xenotropic murine leukemia virus (XMRV), named the CAE (cytoplasmic accumulation element). The CAE enhanced the cytoplasmic accumulation of viral RNA transcripts and the expression of viral proteins without significantly affecting the stability, splicing,

SUBMITTER: Sakuma T 

PROVIDER: S-EPMC3993726 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature